验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, HeLa cells, human brain tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11753-1-AP targets TMOD4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat, Branchiostoma belcheri, zebrafish, Xenopus |
| 免疫原 | TMOD4 fusion protein Ag2349 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tropomodulin 4 (muscle) |
| 别名 | SK TMOD, Skeletal muscle tropomodulin, TMOD4, Tropomodulin 4, tropomodulin 4 (muscle) |
| 计算分子量 | 345 aa, 39 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC017810 |
| 基因名称 | TMOD4 |
| Gene ID (NCBI) | 29765 |
| RRID | AB_2205433 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZQ9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMOD4 is a member of tropomodulin (Tmod) family proteins which are capping proteins binding to the pointed end of actin filaments and preventing both elongation and depolymerization. Four mammalian Tmod genes have been identified to date. Tomd1 is expressed in terminally-differentiated post-mitotic cells, like heart, brain, lens, vasculature, and erythrocytes; Tmod2 expressed in neurons; Tmod3 expressed ubiquitously; Tmod4 expressed only in skeletal muscles. This antibody was raised against the full-length human TMOD4 and may cross-react with other Tmods.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMOD4 antibody 11753-1-AP | Download protocol |
| IHC protocol for TMOD4 antibody 11753-1-AP | Download protocol |
| IP protocol for TMOD4 antibody 11753-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Downsizing the molecular spring of the giant protein titin reveals that skeletal muscle titin determines passive stiffness and drives longitudinal hypertrophy. | ||
J Cell Sci Leiomodin 3 and Tropomodulin 4 have overlapping functions during skeletal myofibrillogenesis. | ||
Dis Model Mech Loss of tropomodulin4 in the zebrafish mutant träge causes cytoplasmic rod formation and muscle weakness reminiscent of nemaline myopathy. | ||
J Proteome Res Proteomic and Phosphoproteomic Analysis of Right Ventricular Hypertrophy in the Pulmonary Hypertension Rat Model | ||
Front Physiol Titin isoform size is not correlated with thin filament length in rat skeletal muscle. |
