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验证数据展示

  • IHC staining of human cervical cancer using 17953-1-AP

    IHC staining of human cervical cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 17953-1-AP

    IHC staining of human cervical cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse skin using 17953-1-AP

    WB analysis of mouse skin using 17953-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 17953-1-AP

    IHC staining of human skin cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skin cancer using 17953-1-AP

    IHC staining of human skin cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 40x lens).

  • Featured Product
  • KD/KO Validated

TMPRSS3 Polyclonal antibody

TMPRSS3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17953-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Synonyms

TADG12, Serine protease TADG-12, ECHOS1, EC:3.4.21.-, DFNB8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse skin tissue
Positive IHC detected inhuman skin cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17953-1-AP targets TMPRSS3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen TMPRSS3 fusion protein Ag12435 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protease, serine 3
Synonyms TADG12, Serine protease TADG-12, ECHOS1, EC:3.4.21.-, DFNB8
Calculated Molecular Weight 453 aa, 49 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC074846
Gene Symbol TMPRSS3
Gene ID (NCBI) 64699
RRIDAB_2878470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57727
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMPRSS3 antibody 17953-1-APDownload protocol
IHC protocol for TMPRSS3 antibody 17953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Gene Med

TMPRSS3 regulates cell viability and apoptosis processes of HEI-OC1 cells via regulation on circ-Slc4a2, miR-182, and AKT cascade.

Authors - Zhi-Wen Zhang
  • KD Validated
View Article
mouseIF

Mol Ther Methods Clin Dev

Selection of viral capsids and promoters affects the efficacy of rescue of Tmprss3-deficient cochlea

Authors - Ksenia A Aaron
  • KO Validated
View Article