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验证数据展示

  • IHC staining of human cervical cancer using 17953-1-AP

    IHC staining of human cervical cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 17953-1-AP

    IHC staining of human cervical cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse skin using 17953-1-AP

    WB analysis of mouse skin using 17953-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 17953-1-AP

    IHC staining of human skin cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skin cancer using 17953-1-AP

    IHC staining of human skin cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 40x lens).

  • Featured Product
  • KD/KO验证

TMPRSS3 Polyclonal antibody

TMPRSS3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17953-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

TADG12, Serine protease TADG-12, ECHOS1, EC:3.4.21.-, DFNB8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skin tissue
Positive IHC detected inhuman skin cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17953-1-AP targets TMPRSS3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 TMPRSS3 fusion protein Ag12435 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protease, serine 3
别名 TADG12, Serine protease TADG-12, ECHOS1, EC:3.4.21.-, DFNB8
计算分子量 453 aa, 49 kDa
观测分子量 40 kDa
GenBank蛋白编号BC074846
基因名称 TMPRSS3
Gene ID (NCBI) 64699
RRIDAB_2878470
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP57727
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMPRSS3 antibody 17953-1-APDownload protocol
IHC protocol for TMPRSS3 antibody 17953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Gene Med

TMPRSS3 regulates cell viability and apoptosis processes of HEI-OC1 cells via regulation on circ-Slc4a2, miR-182, and AKT cascade.

Authors - Zhi-Wen Zhang
  • KD Validated
View Article
mouseIF

Mol Ther Methods Clin Dev

Selection of viral capsids and promoters affects the efficacy of rescue of Tmprss3-deficient cochlea

Authors - Ksenia A Aaron
  • KO Validated
View Article