验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, mouse brain tissue, HeLa cells, Jurkat cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
27489-1-AP targets TMX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | TMX1 fusion protein Ag26595 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thioredoxin-related transmembrane protein 1 |
| 别名 | Thioredoxin-related transmembrane protein 1, Thioredoxin domain-containing protein 1, PSEC0085, Protein disulfide-isomerase TMX1, PDIA11 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC036460 |
| 基因名称 | TMX1 |
| Gene ID (NCBI) | 81542 |
| RRID | AB_2880886 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H3N1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMX1, one of the few transmembrane members of the family, is the first vascular thiol isomerase with antithrombotic function. It forms functional complexes with the ER lectin calnexin and preferentially intervenes during maturation of cysteine-containing, membrane-associated proteins while ignoring the same cysteine-containing ectodomains if not anchored at the ER membrane. As such, TMX1 is the first example of a topology-specific client protein redox catalyst in living cells(PMID: 30655304). Covalent modification with AMS increases the molecular mass of originally oxidized species by ~0.5 kDa per thiol group, which allows enhanced separation from the reduced form in electrophoresis(PMID: 29123984). TMX1 has 2 bands produces by redox with the MW of 30-32 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMX1 antibody 27489-1-AP | Download protocol |
| IHC protocol for TMX1 antibody 27489-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Redox Biol Protein palmitoylation-mediated palmitic acid sensing causes blood-testis barrier damage via inducing ER stress. | ||
Biochem J Opposing regulation of endoplasmic reticulum retention under stress by ERp44 and PDIA6 | ||
J Biol Chem Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome | ||
J Thromb Haemost A novel role for thioredoxin-related transmembrane protein TMX4 in platelet activation and thrombus formation | ||
Mol Oncol Malformin C preferentially kills glioblastoma stem-like cells via concerted induction of proteotoxic stress and autophagic flux blockade |
