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验证数据展示

  • WB analysis of HeLa using 19838-1-AP

    WB analysis of HeLa using 19838-1-AP

    WB result of TMX2 antibody (19838-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMX2 transfected HeLa cells.

  • WB analysis using 19838-1-AP

    WB analysis using 19838-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19838-1-AP (TMX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 19838-1-AP

    IHC staining of human kidney using 19838-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19838-1-AP (TMX2 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 19838-1-AP

    IHC staining of human kidney using 19838-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19838-1-AP (TMX2 antibody) at dilution of 1:200 (under 40x lens).

  • Featured Product
  • KD/KO验证

TMX2 Polyclonal antibody

TMX2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19838-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CGI 31, My009, PDIA12, PIG26, PSEC0045, TMX2, TXNDC14, UNQ237/PRO270

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, HeLa cells, mouse placenta tissue, mouse testis tissue, rat tissue
Positive IHC detected inhuman kidney tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19838-1-AP targets TMX2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TMX2 fusion protein Ag13828 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thioredoxin-related transmembrane protein 2
别名 CGI 31, My009, PDIA12, PIG26, PSEC0045, TMX2, TXNDC14, UNQ237/PRO270
计算分子量 296 aa, 34 kDa
观测分子量 30 kDa, 34 kDa
GenBank蛋白编号BC000666
基因名称 TMX2
Gene ID (NCBI) 51075
RRIDAB_10642435
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y320
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thioredoxin-related transmembrane proteins (TMXs) are protein disulfide isomerase (PDI) family members and possess a transmembrane region. TMX2 cDNA was first isolated in 2003, and TMX2 mRNA has been found in a variety of human tissues, with the highest levels in the heart, brain, liver, kidney, and pancreas.

实验方案

Product Specific Protocols
WB protocol for TMX2 antibody 19838-1-APDownload protocol
IHC protocol for TMX2 antibody 19838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols