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TMX3 Polyclonal antibody

TMX3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21040-1-AP

Print datasheet

Synonyms

KIAA1830, PDIA13, TMX3, TXNDC10

验证数据展示

  • IHC staining of mouse brain using 21040-1-AP

    IHC staining of mouse brain using 21040-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21040-1-AP (TMX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 21040-1-AP

    IHC staining of mouse brain using 21040-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21040-1-AP (TMX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse brain using 21040-1-AP

    WB analysis of mouse brain using 21040-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21040-1-AP (TMX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 21040-1-AP

    IHC staining of human testis using 21040-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21040-1-AP (TMX3 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 21040-1-AP

    IHC staining of human testis using 21040-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21040-1-AP (TMX3 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human stomach tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21040-1-AP targets TMX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TMX3 fusion protein Ag15376 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thioredoxin-related transmembrane protein 3
Synonyms KIAA1830, PDIA13, TMX3, TXNDC10
Calculated Molecular Weight 454 aa, 52 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC032325
Gene Symbol TMX3
Gene ID (NCBI) 54495
RRIDAB_2878798
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JJ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMX3 antibody 21040-1-APDownload protocol
IHC protocol for TMX3 antibody 21040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols