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TNC/Tenascin-C Recombinant antibody, PBS Only (Capture)

TNC/Tenascin-C Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240844A2

Cat no : 83767-1-PBS

Print datasheet

Synonyms

Tenascin, TNC, Hexabrachion, GP 150-225, GP 150 225

验证数据展示

  • WB analysis of U-87 MG using 83767-1-RR (same clone as 83767-1-PBS)

    WB analysis of U-87 MG using 83767-1-RR (same clone as 83767-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 83767-1-RR (TNC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83767-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00755-3

    Cytometric bead array standard curve of MP00755-3

    Cytometric bead array standard curve of MP00755-3, TNC/Tenascin-C Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83767-1-PBS. Detection antibody: 83767-4-PBS. Standard: Ag27122. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83767-1-RR

    Affinity and Kinetic Characterization of 83767-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83767-1-RR against Human TNC/Tenascin-C were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy TNC/Tenascin-C Ready-To-Use IHC Kit. TNC/Tenascin-C primary antibody included.

产品信息

83767-1-PBS targets TNC/Tenascin-C as part of a matched antibody pair:

MP00755-3: 83767-1-PBS capture and 83767-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen TNC/Tenascin-C fusion protein Ag27122 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tenascin C
Synonyms Tenascin, TNC, Hexabrachion, GP 150-225, GP 150 225
Calculated Molecular Weight 2201 aa, 241 kDa
Observed Molecular Weight220-350 kDa, 190-240 kDa
GenBank Accession NumberBC151843
Gene Symbol Tenascin C
Gene ID (NCBI) 3371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24821
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.