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  • Featured Product
  • KD/KO Validated

TNF-alpha Monoclonal antibody, PBS Only

TNF-alpha Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7B8A11

Cat no : 60291-1-PBS

Print datasheet

Synonyms

TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1

验证数据展示

  • WB analysis using 60291-1-Ig (same clone as 60291-1-PBS)

    WB analysis using 60291-1-Ig (same clone as 60291-1-PBS)

    PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody 60004-1-Ig as loading control. This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 60291-1-Ig (same clone as 60291-1-PBS)

    WB analysis of HeLa using 60291-1-Ig (same clone as 60291-1-PBS)

    WB result of TNF alpha antibody (60291-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TNF alpha transfected HeLa cells. This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

    IHC staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60291-1-Ig (TNF Alpha antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNF antibody included
  • IHC staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

    IHC staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60291-1-Ig (TNF Alpha antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNF antibody included
  • IF Staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

    IF Staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60291-1-Ig (TNF alpha antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

    IF Staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60291-1-Ig (TNF alpha antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TNF Ready-To-Use IHC Kit. TNF primary antibody included.

产品信息

60291-1-PBS targets TNF-alpha in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen TNF-alpha fusion protein Ag11413 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor (TNF superfamily, member 2)
Synonyms TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1
Calculated Molecular Weight 233 aa, 26 kDa
Observed Molecular Weight 17-22 kDa
GenBank Accession NumberBC028148
Gene Symbol TNF-alpha
Gene ID (NCBI) 7124
ENSEMBL Gene IDENSG00000232810
RRIDAB_2833255
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01375
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.