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TNF beta Polyclonal antibody

TNF beta Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31505-1-AP

Print datasheet

Synonyms

LTA, LT alpha, TNFB, TNF β, Lymphotoxin-alpha

验证数据展示

  • WB analysis of mouse spleen using 31505-1-AP

    WB analysis of mouse spleen using 31505-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 31505-1-AP (TNF Beta antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse spleen tissue, rat spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31505-1-AP targets TNF beta in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lymphotoxin alpha (TNF superfamily, member 1)
Synonyms LTA, LT alpha, TNFB, TNF β, Lymphotoxin-alpha
Calculated Molecular Weight 205 aa, 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC034729
Gene Symbol TNF beta
Gene ID (NCBI) 4049
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP01374
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF Beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF Beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.

实验方案

Product Specific Protocols
WB protocol for TNF beta antibody 31505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols