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TNF-alpha Recombinant antibody, PBS Only (Capture)

TNF-alpha Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240668E6

Cat no : 83721-5-PBS

Print datasheet

Synonyms

TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1

验证数据展示

  • Sandwich ELISA standard curve of MP00702-4

    Sandwich ELISA standard curve of MP00702-4

    Sandwich ELISA standard curve of MP00702-4, TNF-alpha Recombinant Matched Antibody Pair - PBS only. 83721-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0816. 83721-6-PBS was HRP conjugated as the detection antibody. Range: 3.9-250 pg/mL.

  • Sample test of MP00702-4

    Sample test of MP00702-4

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean TNF-alpha concentration was determined to be 1.3 pg/mL in unstimulated PBMC supernatant,1236.3 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP00702-4

    Sample test of MP00702-4

    Serum of eight individual healthy human donors was measured. The human TNF-alpha concentration of detected samples was determined to be 1.76 pg/mL with a range of 0.72 - 2.78 pg/mL.

  • Cytometric bead array standard curve of MP00702-2

    Cytometric bead array standard curve of MP00702-2

    Cytometric bead array standard curve of MP00702-2, TNF-alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83721-5-PBS. Detection antibody: 83721-4-PBS. Standard: Eg0816. Range: 0.313-40 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy TNF Ready-To-Use IHC Kit. TNF primary antibody included.

产品信息

83721-5-PBS targets TNF-alpha as part of a matched antibody pair:

MP00702-2: 83721-5-PBS capture and 83721-4-PBS detection (validated in Cytometric bead array)

MP00702-4: 83721-5-PBS capture and 83721-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor (TNF superfamily, member 2)
Synonyms TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1
Calculated Molecular Weight26 kDa
GenBank Accession NumberNM_000594.4
Gene Symbol TNF-alpha
Gene ID (NCBI) 7124
ENSEMBL Gene IDENSG00000232810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01375
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C