TNF-alpha Recombinant antibody
TNF-alpha Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240668B4
验证数据展示
经过测试的应用
Positive WB detected in | THP-1 cells, LPS treated THP-1 cells |
Planning an IHC experiment? We recommend our IHCeasy TNF Ready-To-Use IHC Kit. TNF primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 83721-7-RR is TNF-alpha Fusion Protein expressed in E. coli.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Fusion Protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | tumor necrosis factor (TNF superfamily, member 2) |
Synonyms | TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1 |
Calculated Molecular Weight | 26 kDa |
Observed Molecular Weight | 26 kDa |
GenBank Accession Number | NM_000594.4 |
Gene Symbol | TNF-alpha |
Gene ID (NCBI) | 7124 |
ENSEMBL Gene ID | ENSG00000232810 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P01375 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.
实验方案
Product Specific Protocols | |
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WB protocol for TNF-alpha antibody 83721-7-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |