TNF Alpha Polyclonal antibody
TNF Alpha Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (4)
Applications
WB, ELISA and More (4)
Conjugate
Unconjugated
554
验证数据展示
经过测试的应用
Positive WB detected in | THP-1 cells, LPS treated RAW 264.7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 453 publications below |
ELISA | See 3 publications below |
产品信息
17590-1-AP targets TNF Alpha in WB, IF, IHC, ELISA, Cell treatment, Transwell Assay applications and shows reactivity with human, mouse samples.
Tested Applications | WB, ELISA |
Cited Applications | WB, IF, IHC, ELISA, Cell treatment, Transwell Assay |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, rabbit, fish, bovine |
Immunogen | TNF Alpha fusion protein Ag11433 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | tumor necrosis factor (TNF superfamily, member 2) |
Synonyms | Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor |
Calculated Molecular Weight | 233 aa, 26 kDa |
Observed Molecular Weight | 26 kDa |
GenBank Accession Number | BC028148 |
Gene Symbol | TNF |
Gene ID (NCBI) | 7124 |
ENSEMBL Gene ID | ENSG00000232810 |
RRID | AB_2271853 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P01375 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TNF Alpha antibody 17590-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Brain Behav Immun HMGB1 mediates depressive behavior induced by chronic stress through activating the kynurenine pathway. | ||
Bioact Mater A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds | ||
ACS Cent Sci Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury | ||
Small M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation | ||
Mater Today Bio Dexamethasone-loaded zeolitic imidazolate frameworks nanocomposite hydrogel with antibacterial and anti-inflammatory effects for periodontitis treatment | ||
Acta Biomater Mild hyperthermia promotes immune checkpoint blockade-based immunotherapy against metastatic pancreatic cancer using size-adjustable nanoparticles. |