TNFAIP3 Polyclonal antibody

TNFAIP3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 23456-1-AP

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Synonyms

OTUD7C, OTU domain-containing protein 7C, EC:2.3.2.-, A20p50, A20p37



经过测试的应用

Positive WB detected inJurkat cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy TNFAIP3 Ready-To-Use IHC Kit. TNFAIP3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23456-1-AP targets TNFAIP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen TNFAIP3 fusion protein Ag18150 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor necrosis factor, alpha-induced protein 3
Synonyms OTUD7C, OTU domain-containing protein 7C, EC:2.3.2.-, A20p50, A20p37
Calculated Molecular Weight 790 aa, 90 kDa
Observed Molecular Weight 80-90 kDa, 37-40 kDa
GenBank Accession NumberBC114480
Gene Symbol TNFAIP3
Gene ID (NCBI) 7128
RRIDAB_2879284
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21580
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Upon T- or B-cell receptor engagement human (h) A20 is cleaved by MALT1 after arginine 439, yielding an N-terminal fragment and a C-terminal one (~37-40 kDa). This antibody, which is a rabbit polyclonal antibody raised against 350 aa of C-terminus of human TNFAIP3, can recognize full-length A20 (~90 kDa) and cleaved form (~37-40 kDa).

实验方案

Product Specific Protocols
WB protocol for TNFAIP3 antibody 23456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Total Environ

USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.

Authors - Lei Zhang
human,mouseWB,IHC

Am J Cancer Res

ZY-444 inhibits the growth and metastasis of prostate cancer by targeting TNFAIP3 through TNF signaling pathway

Authors - Meng-Ting Han
mouseWB

Biomedicines

The Cholinergic Anti-Inflammatory Pathway Attenuates the Development of Atherosclerosis in Apoe-/- Mice through Modulating Macrophage Functions.

Authors - Zhengjiang Qian

Biomedicines

Sertoli Cells Express Accommodation, Survival, and Immunoregulatory Factors When Exposed to Normal Human Serum

Authors - Rachel L Washburn
ratWB

Int Immunopharmacol

TNFAIP3-upregulated RIP3 exacerbates acute pancreatitis via activating NLRP3 inflammasome.

Authors - Yifan Wang
humanWB

Mol Cell Biol

LncRNA HOXA-AS3 integrates NF-κB signaling to regulate endothelium inflammation.

Authors - Xinxing Zhu