验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, PC-13 cells, A549 cells, MOLT-4 cells |
| Positive IHC detected in | human lung cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15790-1-AP targets TNFAIP8 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | TNFAIP8 fusion protein Ag8491 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor, alpha-induced protein 8 |
| 别名 | GG2 1, MDC 3.13, NDED, SCC S2, SCCS2, TNF alpha induced protein 8, TNF induced protein GG2 1, TNFAIP8 |
| 计算分子量 | 198 aa, 23 kDa |
| 观测分子量 | 19-23 kDa |
| GenBank蛋白编号 | BC007014 |
| 基因名称 | TNFAIP8 |
| Gene ID (NCBI) | 25816 |
| RRID | AB_10792805 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95379 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNFAIP8 (tumor necrosis factor, alpha-induced protein 8), also called SCC-S2/GG2-1/NDED, is associated with enhanced cell survival and inhibition of apoptosis. The induction of TNFAIP8 by TNF depends on the activation of NFκB. TNFAIP8 suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation. TNFAIP8 is expressed in adult spleen, lymph node, thymus, thyroid, bone marrow, and placenta, and in fetal liver, lung, and kidney at high levels, also expressed in various tumor tissues and all cancer cell lines tested. There are four major isoforms of TNFAIP8.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TNFAIP8 antibody 15790-1-AP | Download protocol |
| IHC protocol for TNFAIP8 antibody 15790-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Immunol Crucial Roles of TNFAIP8 Protein in Regulating Apoptosis and Listeria Infection. | ||
Oncotarget TNFAIP8 promotes prostate cancer cell survival by inducing autophagy.
| ||
Toxins (Basel) Cyanotoxins Increase Cytotoxicity and Promote Nonalcoholic Fatty Liver Disease Progression by Enhancing Cell Steatosis | ||
J Proteome Res Proteomic Analysis of Plasma sEVs Reveals That TNFAIP8 Is a New Biomarker of Cell Proliferation in Diabetic Retinopathy.
| ||
Sci Rep The TIPE Molecular Pilot That Directs Lymphocyte Migration in Health and Inflammation.
| ||
Sci Rep The Tnfaip8-PE complex is a novel upstream effector in the anti-autophagic action of insulin.
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