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TNFAIP8 Monoclonal antibody

TNFAIP8 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D2F7

Cat no : 67737-1-Ig

Print datasheet

Synonyms

GG2 1, MDC 3.13, NDED, SCC S2, SCCS2, TNF alpha induced protein 8, TNF induced protein GG2 1, TNFAIP8

验证数据展示

  • WB analysis using 67737-1-Ig

    WB analysis using 67737-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67737-1-Ig (TNFAIP8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, THP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67737-1-Ig targets TNFAIP8 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen TNFAIP8 fusion protein Ag8805 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor, alpha-induced protein 8
Synonyms GG2 1, MDC 3.13, NDED, SCC S2, SCCS2, TNF alpha induced protein 8, TNF induced protein GG2 1, TNFAIP8
Calculated Molecular Weight 198 aa, 23 kDa
Observed Molecular Weight19-21 kDa
GenBank Accession NumberBC007014
Gene Symbol TNFAIP8
Gene ID (NCBI) 25816
RRIDAB_2918506
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95379
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNFAIP8 (tumor necrosis factor, alpha-induced protein 8), also called SCC-S2/GG2-1/NDED, is associated with enhanced cell survival and inhibition of apoptosis. The induction of TNFAIP8 by TNF depends on the activation of NFκB. TNFAIP8 suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation. TNFAIP8 is expressed in adult spleen, lymph node, thymus, thyroid, bone marrow, and placenta, and in fetal liver, lung, and kidney at high levels, also expressed in various tumor tissues and all cancer cell lines tested.

实验方案

Product Specific Protocols
WB protocol for TNFAIP8 antibody 67737-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols