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Host
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Species Reactivity
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0
Conjugate
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验证数据展示

  • WB analysis using 60192-1-Ig (same clone as 60192-1-PBS)

    WB analysis using 60192-1-Ig (same clone as 60192-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1/CD120a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    IF Staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TNFR1/CD120a antibody (60192-1-Ig, Clone: 2A6E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    IHC staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60192-1-Ig (TNFR1/CD120a Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    IHC staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TNFR1/CD120a Monoclonal antibody, PBS Only

TNFR1/CD120a Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60192-1-PBS

产品说明书

CloneNo. 2A6E3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, ELISA

别名

TNFRSF1A, p60, p55 R, p55, FPF

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60192-1-PBS targets TNFR1/CD120a in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 TNFR1/CD120a fusion protein Ag16112 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 1A
别名 TNFRSF1A, p60, p55 R, p55, FPF
计算分子量 455 aa, 50 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC010140
基因名称 TNFR1
Gene ID (NCBI) 7132
RRIDAB_10897160
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19438
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.