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  • Featured Product
  • KD/KO Validated

TNFR1 Monoclonal antibody, PBS Only

TNFR1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A6E3

Cat No : 60192-1-PBS

Print datasheet

Synonyms

CD120a, FPF, p55, p55 R, p60, TBP1, TBPI, TNF R, TNF R I, TNF R1, TNF R55, TNF Receptor 1, TNF Receptor I, TNF RI, TNFAR, TNFR I, TNFR1, TNFR55, TNFR60, TNFRSF1A

验证数据展示

  • WB analysis using 60192-1-Ig (same clone as 60192-1-PBS)

    WB analysis using 60192-1-Ig (same clone as 60192-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    IF Staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TNFR1 antibody (60192-1-Ig, Clone: 2A6E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    IHC staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    IHC staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

产品信息

60192-1-PBS targets TNFR1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen TNFR1 fusion protein Ag16112 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 1A
Synonyms CD120a, FPF, p55, p55 R, p60, TBP1, TBPI, TNF R, TNF R I, TNF R1, TNF R55, TNF Receptor 1, TNF Receptor I, TNF RI, TNFAR, TNFR I, TNFR1, TNFR55, TNFR60, TNFRSF1A
Calculated Molecular Weight 455 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC010140
Gene Symbol TNFRSF1A
Gene ID (NCBI) 7132
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19438
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.