CoraLite® Plus 488-conjugated TNFR2 Polyclonal antibody
TNFR2 Polyclonal Antibody for FC
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
Planning an IHC experiment? We recommend our IHCeasy TNFR2/TNFRSF1B Ready-To-Use IHC Kit. TNFR2/TNFRSF1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 28746-1-AP
产品信息
CL488-28746 targets TNFR2 in FC applications and shows reactivity with Human samples.
Tested Applications | FC Application Description |
Tested Reactivity | Human |
Immunogen | TNFR2 fusion protein Ag30490 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | tumor necrosis factor receptor superfamily, member 1B |
Synonyms | CD120b, Etanercept, p75, p75TNFR, p80 TNF alpha receptor, TBP 2, TBPII, TNF R II, TNF R2, TNF R75, TNF RII, TNFBR, TNFR II, TNFR1B, TNFR2, TNFR80, TNFRSF1B |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 75 kDa, 65 kDa |
GenBank Accession Number | BC052977 |
Gene Symbol | TNFRSF1B |
Gene ID (NCBI) | 7133 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P20333 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 TNFR2 antibody CL488-28746 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |