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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 28746-1-AP (same clone as 28746-1-PBS)

    WB analysis using 28746-1-AP (same clone as 28746-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 28746-1-AP (same clone as 28746-1-PBS)

    IHC staining of human tonsillitis using 28746-1-AP (same clone as 28746-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNFR2/TNFRSF1B antibody included
  • IHC staining of human tonsillitis using 28746-1-AP (same clone as 28746-1-PBS)

    IHC staining of human tonsillitis using 28746-1-AP (same clone as 28746-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28746-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNFR2/TNFRSF1B antibody included
  • IF Staining of THP-1 using 28746-1-AP (same clone as 28746-1-PBS)

    IF Staining of THP-1 using 28746-1-AP (same clone as 28746-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TNFR2 / TNFRSF1B antibody (28746-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28746-1-PBS in a different storage buffer formulation.

TNFR2 / TNFRSF1B Polyclonal antibody, PBS Only

TNFR2 / TNFRSF1B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28746-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TNFR2/TNFRSF1B Ready-To-Use IHC Kit. TNFR2/TNFRSF1B primary antibody included.

产品信息

28746-1-PBS targets TNFR2 / TNFRSF1B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 TNFR2 / TNFRSF1B fusion protein Ag30490 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 1B
别名
计算分子量 48 kDa
观测分子量 75 kDa, 65 kDa
GenBank蛋白编号BC052977
基因名称 TNFR2
Gene ID (NCBI) 7133
RRIDAB_2881205
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20333
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).