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验证数据展示

  • WB analysis of human milk using 11534-1-AP

    WB analysis of human milk using 11534-1-AP

    human milk was subjected to SDS PAGE followed by western blot with 11534-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 11534-1-AP

    IHC staining of rat kidney using 11534-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11534-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 11534-1-AP

    IHC staining of rat kidney using 11534-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11534-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 11534-1-AP

    IF Staining of HepG2 using 11534-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Osteoprotegerin antibody (11534-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Osteoprotegerin/TNFRSF11B Polyclonal antibody

Osteoprotegerin/TNFRSF11B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11534-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

Tumor necrosis factor receptor superfamily member 11B, TNFRSF11B, Osteoprotegerin, Osteoclastogenesis inhibitory factor, OPG

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman milk
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11534-1-AP targets Osteoprotegerin/TNFRSF11B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, rat
免疫原 Osteoprotegerin/TNFRSF11B fusion protein Ag2139 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 11b
别名 Tumor necrosis factor receptor superfamily member 11B, TNFRSF11B, Osteoprotegerin, Osteoclastogenesis inhibitory factor, OPG
计算分子量 401 aa, 46 kDa
观测分子量56 kDa
GenBank蛋白编号BC030155
基因名称 TNFRSF11B
Gene ID (NCBI) 4982
RRIDAB_3085376
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00300
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Osteoprotegerin (OPG), also known as tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) and osteoclastogenesis inhibitory factor (OCIF), belongs to the tumor necrosis factor (TNF) receptor superfamily. By binding to RANKL, OPG inhibits osteoclastic activity and promoting bone formationis. OPG plays a key role on cell survival by inhibiting TRAIL-induced apoptosis. OPG also is involved in vascular biology, fibrosis, immunity, EMT, and the apoptosis of cancer cells (PMID: 35523775).

实验方案

Product Specific Protocols
WB protocol for Osteoprotegerin/TNFRSF11B antibody 11534-1-APDownload protocol
IHC protocol for Osteoprotegerin/TNFRSF11B antibody 11534-1-APDownload protocol
IF protocol for Osteoprotegerin/TNFRSF11B antibody 11534-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Anal Cell Pathol (Amst)

Cartilage Damage Pathological Characteristics of Diabetic Neuropathic Osteoarthropathy

Authors - Pei-Long Liu
View Article
rat,humanIHC

BMC Oral Health

Artesunate-loaded thermosensitive chitosan hydrogel promotes osteogenesis of maxillary tooth extraction through regulating T lymphocytes in type 2 diabetic rats

Authors - Jinghong Luo
View Article
WB

Foods

Optimization of the Preparation Process and Ameliorative Efficacy in Osteoporotic Rats of Peptide-Calcium Chelates from Skipjack Tuna (Katsuwonus pelamis) Meat

Authors - Wan-Zhen Yan
View Article