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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 32133-1-AP

    WB analysis using 32133-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32133-1-AP (4-1BB/TNFRSF9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

TNFRSF9/CD137 Polyclonal antibody

TNFRSF9/CD137 Polyclonal Antibody for WB, ELISA
Cat No. 32133-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

T-cell antigen 4-1BB, Ly63, Ila, CD137, 4-1BB ligand receptor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inCTLL-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32133-1-AP targets TNFRSF9/CD137 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 9
Synonyms T-cell antigen 4-1BB, Ly63, Ila, CD137, 4-1BB ligand receptor
Calculated Molecular Weight28 kDa
Observed Molecular Weight28-30 kDa, 40-45 kDa
GenBank Accession NumberNM_001077509.1
Gene Symbol Tnfrsf9
Gene ID (NCBI) 21942
RRIDAB_3670203
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP20334
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)

实验方案

Product Specific Protocols
WB protocol for TNFRSF9/CD137 antibody 32133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols