验证数据展示
经过测试的应用
Positive WB detected in | CTLL-2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:16000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
32133-1-AP targets TNFRSF9/CD137 in WB, ELISA applications and shows reactivity with mouse samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | mouse |
Immunogen | Recombinant protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | tumor necrosis factor receptor superfamily, member 9 |
Synonyms | T-cell antigen 4-1BB, Ly63, Ila, CD137, 4-1BB ligand receptor |
Calculated Molecular Weight | 28 kDa |
Observed Molecular Weight | 28-30 kDa, 40-45 kDa |
GenBank Accession Number | NM_001077509.1 |
Gene Symbol | Tnfrsf9 |
Gene ID (NCBI) | 21942 |
RRID | AB_3670203 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
UNIPROT ID | P20334 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)
实验方案
Product Specific Protocols | |
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WB protocol for TNFRSF9/CD137 antibody 32133-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |