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验证数据展示

  • WB analysis of U-937 using 11862-1-AP

    WB analysis of U-937 using 11862-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 11862-1-AP (TNFSF13B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 11862-1-AP

    WB analysis using 11862-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11862-1-AP (TNFSF13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 11862-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 11862-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11862-1-AP (TNFSF13B/BAFF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BAFF/TNFSF13B Polyclonal antibody

BAFF/TNFSF13B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11862-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

TNFSF13B, CD257, BLYS, B-cell-activating factor, BAFF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-937 cells, mouse testis tissue, mouse liver tissue, human liver tissue, rat liver tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11862-1-AP targets BAFF/TNFSF13B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BAFF/TNFSF13B fusion protein Ag2440 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor necrosis factor (ligand) superfamily, member 13b
别名 TNFSF13B, CD257, BLYS, B-cell-activating factor, BAFF
计算分子量 32 kDa
观测分子量 32 kDa, 45-52 kDa
GenBank蛋白编号BC020674
基因名称 TNFSF13B
Gene ID (NCBI) 10673
RRIDAB_2303658
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y275
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNFSF13B, also known as BAFF, BLYS, TALL1, TNFSF20, ZTNF4 and CD257, is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. This cytokine is expressed in B lineage cells, and acts as a potent B cell activator. The BAFF system promotes B cell survival and differentiation and plays a prominent role in the pathogenesis of autoimmune diseases. It has been shown that BAFF inhibitors are in clinical trials for systemic lupus erythematosus with significant efficacy. This antibody recognizes all the forms of TNFSF13B such as soluble form (17 kDa), membrane form (32-35 kDa) and glycosylation form (45-52 kDa).

实验方案

Product Specific Protocols
WB protocol for BAFF/TNFSF13B antibody 11862-1-APDownload protocol
IHC protocol for BAFF/TNFSF13B antibody 11862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cancer Sci

Spheroid culture to select theoretical therapeutic drugs in intravascular large B-cell lymphoma

Authors - Mika Takai
View Article