• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TNFSF8 Polyclonal antibody

TNFSF8 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 17852-1-AP

Print datasheet

Synonyms

CD153, CD30 L, CD30 ligand, CD30L, CD30LG, TNFSF8

验证数据展示

  • IHC staining of human liver cancer using 17852-1-AP

    IHC staining of human liver cancer using 17852-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17852-1-AP (TNFSF8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 17852-1-AP

    IHC staining of human liver cancer using 17852-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17852-1-AP (TNFSF8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17852-1-AP targets TNFSF8 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TNFSF8 fusion protein Ag12303 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor necrosis factor (ligand) superfamily, member 8
Synonyms CD153, CD30 L, CD30 ligand, CD30L, CD30LG, TNFSF8
Calculated Molecular Weight 234 aa, 26 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC093630
Gene Symbol TNFSF8
Gene ID (NCBI) 944
RRIDAB_2878452
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32971
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNFSF8, also named as CD30LG and CD153, is a member of the tumor necrosis factor (TNF) receptor superfamily, is a surface antigen used as a clinical marker for Hodgkin lymphoma and related hematologic malignancies. It induces proliferation of T-cells. CD30L enhanced the proliferation of CD3-activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines. For Glycosylation, the MW in WB detection is about 35-40 kDa.

实验方案

Product Specific Protocols
WB protocol for TNFSF8 antibody 17852-1-APDownload protocol
IHC protocol for TNFSF8 antibody 17852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols