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验证数据展示

  • IHC staining of human liver cancer using 17852-1-AP

    IHC staining of human liver cancer using 17852-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17852-1-AP (TNFSF8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 17852-1-AP

    IHC staining of human liver cancer using 17852-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17852-1-AP (TNFSF8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TNFSF8 Polyclonal antibody

TNFSF8 Polyclonal Antibody for IHC, ELISA
Cat No. 17852-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA

别名

CD153, CD30 L, CD30 ligand, CD30L, CD30LG, TNFSF8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17852-1-AP targets TNFSF8 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TNFSF8 fusion protein Ag12303 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor necrosis factor (ligand) superfamily, member 8
别名 CD153, CD30 L, CD30 ligand, CD30L, CD30LG, TNFSF8
计算分子量 234 aa, 26 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC093630
基因名称 TNFSF8
Gene ID (NCBI) 944
RRIDAB_2878452
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32971
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNFSF8, also named as CD30LG and CD153, is a member of the tumor necrosis factor (TNF) receptor superfamily, is a surface antigen used as a clinical marker for Hodgkin lymphoma and related hematologic malignancies. It induces proliferation of T-cells. CD30L enhanced the proliferation of CD3-activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines. For Glycosylation, the MW in WB detection is about 35-40 kDa.

实验方案

Product Specific Protocols
WB protocol for TNFSF8 antibody 17852-1-APDownload protocol
IHC protocol for TNFSF8 antibody 17852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols