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验证数据展示

  • WB analysis using 13955-1-AP

    WB analysis using 13955-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13955-1-AP (TNIK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

TNIK Polyclonal antibody

TNIK Polyclonal Antibody for WB, ELISA
Cat No. 13955-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

TRAF2 and NCK-interacting protein kinase, KIAA0551, EC:2.7.11.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, COLO 205 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13955-1-AP targets TNIK in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 TNIK fusion protein Ag5068 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TRAF2 and NCK interacting kinase
别名 TRAF2 and NCK-interacting protein kinase, KIAA0551, EC:2.7.11.1
计算分子量 155 kDa
观测分子量150~180 kDa
GenBank蛋白编号BC055427
基因名称 TNIK
Gene ID (NCBI) 23043
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKE5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNIK (Traf2- and Nck-interacting kinase) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension, which had been implicated in postsynaptic signaling as well as in regulation of cell proliferation. TNiK is expressed in the nervous system and plays key roles at the synapse and nucleus, In non-neuronal cells, TNiK has been described as a critical component of transcriptional regulatory mechanisms, mediated by Wnt signaling (PMID: 24566388). TNIK is also an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462). Western blot analysis detected TNIK at an apparent molecular mass of 150~180 kDa as cited in literature (PMID: 24566388).

实验方案

Product Specific Protocols
WB protocol for TNIK antibody 13955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols