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TNIK Polyclonal antibody

TNIK Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 30906-1-AP

Print datasheet

Synonyms

KIAA0551, TNIK

验证数据展示

  • WB analysis using 30906-1-AP

    WB analysis using 30906-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30906-1-AP (TNIK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 30906-1-AP

    IF Staining of K-562 using 30906-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using TNIK antibody (30906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inK-562 cells, HT-29 cells
Positive IF detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30906-1-AP targets TNIK in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen TNIK fusion protein Ag34132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TRAF2 and NCK interacting kinase
Synonyms KIAA0551, TNIK
Observed Molecular Weight170 kDa
GenBank Accession NumberBC150256
Gene Symbol TNIK
Gene ID (NCBI) 23043
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UKE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Traf2 and NcK interacting kinase (TNiK), a protein with both scaffolding and kinase domains, had been implicated in postsynaptic signaling as well as in regulation of cell proliferation. TNiK is expressed in the nervous system and plays key roles at the synapse and nucleus, In non-neuronal cells, TNiK has been described as a critical component of transcriptional regulatory mechanisms, mediated by Wnt signaling.

实验方案

Product Specific Protocols
WB protocol for TNIK antibody 30906-1-APDownload protocol
IF protocol for TNIK antibody 30906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols