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TNIK Monoclonal antibody, PBS Only

TNIK Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E9H3

Cat no : 67948-1-PBS

Print datasheet

Synonyms

TNIK, TRAF2 and NCK-interacting protein kinase, 1E9H3, EC:2.7.11.1, KIAA0551

验证数据展示

  • WB analysis of K-562 using 67948-1-Ig (same clone as 67948-1-PBS)

    WB analysis of K-562 using 67948-1-Ig (same clone as 67948-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • WB analysis of human peripheral blood platelets using 67948-1-Ig (same clone as 67948-1-PBS)

    WB analysis of human peripheral blood platelets using 67948-1-Ig (same clone as 67948-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67948-1-Ig (same clone as 67948-1-PBS)

    IHC staining of human kidney using 67948-1-Ig (same clone as 67948-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67948-1-Ig (same clone as 67948-1-PBS)

    IHC staining of human kidney using 67948-1-Ig (same clone as 67948-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 67948-1-Ig (same clone as 67948-1-PBS)

    IF Staining of A431 using 67948-1-Ig (same clone as 67948-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TNIK antibody (67948-1-Ig, Clone: 1E9H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 67948-1-Ig (same clone as 67948-1-PBS)

    FC experiment of A431 using 67948-1-Ig (same clone as 67948-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TNIK (67948-1-Ig, Clone:1E9H3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

产品信息

67948-1-PBS targets TNIK in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity Human
Immunogen TNIK fusion protein Ag30710 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name TRAF2 and NCK interacting kinase
Synonyms TNIK, TRAF2 and NCK-interacting protein kinase, 1E9H3, EC:2.7.11.1, KIAA0551
Calculated Molecular Weight 155 kDa
GenBank Accession NumberBC055427
Gene Symbol TNIK
Gene ID (NCBI) 23043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKE5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C