验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, mouse skeletal muscle tissue, HEK-293 cells, HeLa cells, U2OS cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy TNIP1 Ready-To-Use IHC Kit. TNIP1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15104-1-AP targets TNIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | TNIP1 fusion protein Ag1561 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TNFAIP3 interacting protein 1 |
| 别名 | ABIN1, hVAN, HIV-1 Nef-interacting protein, ABIN-1, ABIN 1 |
| 计算分子量 | 72 kDa |
| 观测分子量 | 72 kDa |
| GenBank蛋白编号 | BC014008 |
| 基因名称 | TNIP1 |
| Gene ID (NCBI) | 10318 |
| RRID | AB_2207757 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15025 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNIP1, also named as KIAA0113, NAF1, Nip40-1, VAN and ABIN-1, is involved in inhibition of nuclear factor- B (NF- B) activation by interacting with TNF alpha-induced protein 3, A20 (TNFAIP3). TNIP1 repression of RARs in the presence of RA places it in a small category of corepressors of agonist-bound NRs.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TNIP1 antibody 15104-1-AP | Download protocol |
| IHC protocol for TNIP1 antibody 15104-1-AP | Download protocol |
| IF protocol for TNIP1 antibody 15104-1-AP | Download protocol |
| IP protocol for TNIP1 antibody 15104-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1 | ||
Cell Death Differ Ubiquitin-binding domain in ABIN1 is critical for regulating cell death and inflammation during development. | ||
BMC Biol A functional genomics screen for microRNA regulators of NF-kappaB signaling.
| ||
Retrovirology Immune regulator ABIN1 suppresses HIV-1 transcription by negatively regulating the ubiquitination of Tat.
| ||
J Cell Biochem ABIN1 inhibits HDAC1 ubiquitination and protects HDAC1 from both proteasome- and lysozyme-dependent degradation.
| ||
Clin Exp Pharmacol Physiol ABIN-1 protects chondrocytes from lipopolysaccharide-induced inflammatory injury through the inactivation of NF-κB signalling. |
