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TNK1 Polyclonal antibody

TNK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14199-1-AP

Print datasheet

Synonyms

CD38 negative kinase 1, TNK1

验证数据展示

  • WB analysis using 14199-1-AP

    WB analysis using 14199-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14199-1-AP (TNK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 14199-1-AP

    IHC staining of human prostate cancer using 14199-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 14199-1-AP

    IHC staining of human prostate cancer using 14199-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, HL-60 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14199-1-AP targets TNK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen TNK1 fusion protein Ag5402 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tyrosine kinase, non-receptor, 1
Synonyms CD38 negative kinase 1, TNK1
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC035782
Gene Symbol TNK1
Gene ID (NCBI) 8711
RRIDAB_2205700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13470
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TNK1 antibody 14199-1-APDownload protocol
IHC protocol for TNK1 antibody 14199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Immunol

Thirty-Eight-Negative Kinase 1 Is a Mediator of Acute Kidney Injury in Experimental and Clinical Traumatic Hemorrhagic Shock.

Authors - Rebecca Halbgebauer
View Article
human,ratWB, IHC

3 Biotech

Tyrosine kinase nonreceptor 1 (TNK1) knockdown ameliorates hemorrhage shock-induced kidney injury via inhibiting macrophage M1 polarization.

Authors - Miaolong Tang
View Article