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TNN Polyclonal antibody

TNN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24839-1-AP

Print datasheet

Synonyms

tenascin N, TN W, TN N, tenascin-N

验证数据展示

  • WB analysis using 24839-1-AP

    WB analysis using 24839-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24839-1-AP (TNN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24839-1-AP targets TNN in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen TNN fusion protein Ag18127 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tenascin N
Synonyms tenascin N, TN W, TN N, tenascin-N
Calculated Molecular Weight 1299 aa, 144 kDa
Observed Molecular Weight144 kDa, 173 kDa
GenBank Accession NumberBC136619
Gene Symbol TNN
Gene ID (NCBI) 63923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQP3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNN (Tenascin-W), also known as TNW. It is expected to be located in the extracellular space. The protein is not detected in normal adult mammary tissues or brain but expressed in most breast tumors and brain tumors, such as glioblastomas, astrocytomas and oligodendrogliomas, tested (PMID:17909022). And in brain tumors, detected around the endothelial cell layer of the clood vessels (PMID: 19884327). TNN is the fourth and final member of the extracellular matrix protein family of tenascins. In vitro, the presence of tenascin-W increased the proportion of elongated human umbilical vein endothelial cells (HUVECs) and augmented the mean speed of cell migration. Furthermore, tenascin-W triggered sprouting of HUVEC spheroids to a similar extent as the proangiogenic factor tenascin-C. Tenascin-W is a specific marker of glioma-associated blood vessels and stimulates angiogenesis in vitro(PMID: 19884327). The predicted molecular weight of the protein is 144 kDa in human and 173 kDa in mouse.

实验方案

Product Specific Protocols
WB protocol for TNN antibody 24839-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols