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验证数据展示

  • WB analysis using 24839-1-AP

    WB analysis using 24839-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24839-1-AP (TNN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TNN Polyclonal antibody

TNN Polyclonal Antibody for WB, ELISA
Cat No. 24839-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

tenascin N, TN W, TN N, tenascin-N

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24839-1-AP targets TNN in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TNN fusion protein Ag18127 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tenascin N
别名 tenascin N, TN W, TN N, tenascin-N
计算分子量 1299 aa, 144 kDa
观测分子量144 kDa, 173 kDa
GenBank蛋白编号BC136619
基因名称 TNN
Gene ID (NCBI) 63923
RRIDAB_3669454
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQP3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNN (Tenascin-W), also known as TNW. It is expected to be located in the extracellular space. The protein is not detected in normal adult mammary tissues or brain but expressed in most breast tumors and brain tumors, such as glioblastomas, astrocytomas and oligodendrogliomas, tested (PMID:17909022). And in brain tumors, detected around the endothelial cell layer of the clood vessels (PMID: 19884327). TNN is the fourth and final member of the extracellular matrix protein family of tenascins. In vitro, the presence of tenascin-W increased the proportion of elongated human umbilical vein endothelial cells (HUVECs) and augmented the mean speed of cell migration. Furthermore, tenascin-W triggered sprouting of HUVEC spheroids to a similar extent as the proangiogenic factor tenascin-C. Tenascin-W is a specific marker of glioma-associated blood vessels and stimulates angiogenesis in vitro(PMID: 19884327). The predicted molecular weight of the protein is 144 kDa in human and 173 kDa in mouse.

实验方案

Product Specific Protocols
WB protocol for TNN antibody 24839-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols