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TNNI1 Polyclonal antibody

TNNI1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22253-1-AP

Print datasheet

Synonyms

SSTNI, TNN1, TNNI1

验证数据展示

  • WB analysis using 22253-1-AP

    WB analysis using 22253-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22253-1-AP (TNNI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 22253-1-AP

    IHC staining of human skeletal muscle using 22253-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 22253-1-AP

    IHC staining of human skeletal muscle using 22253-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22253-1-AP targets TNNI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TNNI1 fusion protein Ag17749 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name troponin I type 1 (skeletal, slow)
Synonyms SSTNI, TNN1, TNNI1
Calculated Molecular Weight 187 aa, 22 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC012600
Gene Symbol TNNI1
Gene ID (NCBI) 7135
RRIDAB_11182276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19237
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TNNI1 antibody 22253-1-APDownload protocol
IHC protocol for TNNI1 antibody 22253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols