TNNI2 Polyclonal antibody

TNNI2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 22130-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, ELISA

Troponin I fast skeletal muscle

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22130-1-AP targets TNNI2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 TNNI2 fusion protein Ag17750 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 troponin I type 2 (skeletal, fast)
别名 Troponin I fast skeletal muscle
计算分子量 182 aa, 21 kDa
观测分子量 21-26 kDa
GenBank蛋白编号BC032148
基因名称 TNNI2
Gene ID (NCBI) 7136
RRIDAB_2879002
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48788
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TNNI2 antibody 22130-1-APDownload protocol
IHC protocol for TNNI2 antibody 22130-1-APDownload protocol
IF protocol for TNNI2 antibody 22130-1-APDownload protocol
IP protocol for TNNI2 antibody 22130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Ann Surg Oncol

Troponin I2 as a Specific Biomarker for Prediction of Peritoneal Metastasis in Gastric Cancer.

Authors - Koichi Sawaki
mouseWB

Sci Rep

A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice.

Authors - Kensuke Ihara
mouseWB

Front Physiol

Lipidomic Analyses Reveal Specific Alterations of Phosphatidylcholine in Dystrophic Mdx Muscle.

Authors - William J Valentine
mouseWB

J Muscle Res Cell Motil

Differential regulation of Actn2 and Actn3 expression during unfolded protein response in C2C12 myotubes.

Authors - Nagakatsu Harada
Loading...