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验证数据展示

  • IF Staining of hTERT-RPE1 using 68631-1-Ig

    IF Staining of hTERT-RPE1 using 68631-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TNNT1 antibody (68631-1-Ig, Clone: 1B1D5 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis using 68631-1-Ig

    WB analysis using 68631-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68631-1-Ig (TNNT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 68631-1-Ig

    IHC staining of mouse skeletal muscle using 68631-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68631-1-Ig (TNNT1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat skeletal muscle using 68631-1-Ig

    IHC staining of rat skeletal muscle using 68631-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68631-1-Ig (TNNT1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat skeletal muscle using 68631-1-Ig

    IHC staining of rat skeletal muscle using 68631-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68631-1-Ig (TNNT1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TNNT1 Monoclonal antibody

TNNT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68631-1-Ig
CloneNo. 1B1D5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TNTS, TNT, STNT, ANM

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IHC detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68631-1-Ig targets TNNT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TNNT1 fusion protein Ag8919 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name troponin T type 1 (skeletal, slow)
Synonyms TNTS, TNT, STNT, ANM
Calculated Molecular Weight252aa,27 kDa; 192aa,21 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC022086
Gene Symbol TNNT1
Gene ID (NCBI) 7138
RRIDAB_3085322
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13805
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TNNT1 antibody 68631-1-IgDownload protocol
IHC protocol for TNNT1 antibody 68631-1-IgDownload protocol
IF protocol for TNNT1 antibody 68631-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols