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TNNT3 Polyclonal antibody

TNNT3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19729-1-AP

Print datasheet

Synonyms

Troponin T-FS, Troponin T3 skeletal fast, Fast skeletal muscle troponin T, Beta-TnTF

验证数据展示

  • WB analysis of rat skeletal muscle using 19729-1-AP

    WB analysis of rat skeletal muscle using 19729-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19729-1-AP (TNNT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 19729-1-AP

    IHC staining of mouse skeletal muscle using 19729-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19729-1-AP (TNNT3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 19729-1-AP

    IHC staining of mouse skeletal muscle using 19729-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19729-1-AP (TNNT3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19729-1-AP targets TNNT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name troponin T type 3 (skeletal, fast)
Synonyms Troponin T-FS, Troponin T3 skeletal fast, Fast skeletal muscle troponin T, Beta-TnTF
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberNM_001042780
Gene Symbol TNNT3
Gene ID (NCBI) 7140
RRIDAB_2878601
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP45378
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TNNT3 antibody 19729-1-APDownload protocol
IHC protocol for TNNT3 antibody 19729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols