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  • Featured Product
  • KD/KO Validated

TNPO2 Polyclonal antibody

TNPO2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 17831-1-AP

Print datasheet

Synonyms

TNPO 2, KPNB2B, Karyopherin beta-2b, IPO3, Importin 3

验证数据展示

  • WB analysis of fetal human brain using 17831-1-AP

    WB analysis of fetal human brain using 17831-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 17831-1-AP

    IF Staining of MCF-7 using 17831-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TNPO2 antibody (17831-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected infetal human brain tissue, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17831-1-AP targets TNPO2 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen TNPO2 fusion protein Ag12229 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transportin 2
Synonyms TNPO 2, KPNB2B, Karyopherin beta-2b, IPO3, Importin 3
Calculated Molecular Weight 887 aa, 100 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC072420
Gene Symbol TNPO2
Gene ID (NCBI) 30000
RRIDAB_10598481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14787
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TNPO2 antibody 17831-1-APDownload protocol
IF protocol for TNPO2 antibody 17831-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Rep

Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function.

Authors - Eva-Maria Hock
View Article
humanWB,IF

Sci Rep

Transportin-2 plays a critical role in nucleocytoplasmic shuttling of oestrogen receptor-α.

Authors - Tetsuji Moriyama
  • KD Validated
View Article
mouseWB

eLife

Kap-β2/Transportin mediates β-catenin nuclear transport in Wnt signaling

Authors - Woong Y Hwang
View Article
humanWB,CoIP

Int J Biol Macromol

Membrane-bound transcription factor LRRC4 inhibits glioblastoma cell motility

Authors - Yang Liu
View Article