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验证数据展示

  • WB analysis using 32165-1-AP

    WB analysis using 32165-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32165-1-AP (TNRC18 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 32165-1-AP

    WB analysis using 32165-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32165-1-AP (TNRC18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

TNRC18 Polyclonal antibody

TNRC18 Polyclonal Antibody for WB, ELISA
Cat No. 32165-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

Trinucleotide repeat-containing gene 18 protein, Trinucleotide Repeat Containing 18, Long CAG trinucleotide repeat-containing gene 79 protein, KIAA1856, CAGL79

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, HepG2 cells, MDA-MB-453 cells, mouse kidney tissue, mouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32165-1-AP targets TNRC18 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 TNRC18 fusion protein Ag37483 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trinucleotide repeat containing 18
别名 Trinucleotide repeat-containing gene 18 protein, Trinucleotide Repeat Containing 18, Long CAG trinucleotide repeat-containing gene 79 protein, KIAA1856, CAGL79
计算分子量31 kDa
观测分子量29 kDa
GenBank蛋白编号BC131808
基因名称 TNRC18
Gene ID (NCBI) 84629
RRIDAB_3670212
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO15417
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Trinucleotide repeat containing 18 (TNRC18, also known as CAGL79 and KIAA1856) is a nucleic-acid-binding protein, which recognizes H3K9me3 to mediate the silencing of ERV class I (ERV1) elements and acts as a chromatin-associated regulator of ERVs (PMID: 27980689; 37938770). It is located in the cytosol, mitochondrion, and nucleus (PMID: 19262598). The mutation in TNRC18 may cause Fazio-Londe disease (PMID: 38188848).

实验方案

Product Specific Protocols
WB protocol for TNRC18 antibody 32165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols