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  • Featured Product
  • KD/KO Validated

TOAG1 Polyclonal antibody

TOAG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21564-1-AP

Print datasheet

Synonyms

C3orf23, TCAIM, T-cell activation inhibitor, TOAG1

验证数据展示

  • IHC staining of human liver using 21564-1-AP

    IHC staining of human liver using 21564-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21564-1-AP (TOAG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HEK-293 using 21564-1-AP

    WB analysis of HEK-293 using 21564-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21564-1-AP (TOAG1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KOSee 1 publications below
IHCSee 1 publications below

产品信息

21564-1-AP targets TOAG1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen TOAG1 fusion protein Ag16067 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 3 open reading frame 23
Synonyms C3orf23, TCAIM, T-cell activation inhibitor, TOAG1
Calculated Molecular Weight 496 aa, 58 kDa
Observed Molecular Weight 35-41 kDa
GenBank Accession NumberBC101060
Gene Symbol TOAG1
Gene ID (NCBI) 285343
RRIDAB_2878885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N3R3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOAG1, also named as TCAIM and C3orf23, is a mitochondrial protein which regulates T-cell apoptosis. It is a specific and reproducible marker for quiescence. TOAG1 has two isoforms with MW 41 kDa and 58 kDa.

实验方案

Product Specific Protocols
WB protocol for TOAG1 antibody 21564-1-APDownload protocol
IHC protocol for TOAG1 antibody 21564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

FEBS J

A novel role of TCAIM: suppressing renal carcinoma growth and enhancing its sensitivity to sunitinib

Authors - Zhuo Ye
  • KD Validated
View Article