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TOB1 Recombinant antibody

TOB1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241043A5

Cat no : 83944-1-RR

Print datasheet

Synonyms

Transducer of erbB 2 1, TOB, Protein Tob1, PIG49, APRO6

验证数据展示

  • WB analysis using 83944-1-RR

    WB analysis using 83944-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83944-1-RR (TOB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83944-1-RR

    Affinity and Kinetic Characterization of 83944-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83944-1-RR against Human TOB1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, MCF-7 cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83944-1-RR targets TOB1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen TOB1 fusion protein Ag6714 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transducer of ERBB2, 1
Synonyms Transducer of erbB 2 1, TOB, Protein Tob1, PIG49, APRO6
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38-45 kDa
GenBank Accession NumberBC031406
Gene Symbol TOB1
Gene ID (NCBI) 10140
RRIDAB_3671523
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50616
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. It is mainly located in the cell nucleus and will transfer between the cell nucleus and the cytoplasm. The more phosphorylation, the more obvious the cytoplasmic localization.The predicted MW of this protein is 38 kDa. Catalog#83944-1-RR recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.

实验方案

Product Specific Protocols
WB protocol for TOB1 antibody 83944-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols