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验证数据展示

  • WB analysis using 13607-1-AP

    WB analysis using 13607-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13607-1-AP (TOB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 13607-1-AP

    IHC staining of human kidney using 13607-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13607-1-AP (TOB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TOB2 antibody included
  • IF Staining of HepG2 using 13607-1-AP

    IF Staining of HepG2 using 13607-1-AP

    Immunofluorescent analysis of HepG2 cells using 13607-1-AP (TOB2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TOB2 Polyclonal antibody

TOB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13607-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Transducer of erbB 2 2, TOBL, TOB4, Protein Tob4, Protein Tob2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TOB2 Ready-To-Use IHC Kit. TOB2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13607-1-AP targets TOB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 TOB2 fusion protein Ag4491 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transducer of ERBB2, 2
别名 Transducer of erbB 2 2, TOBL, TOB4, Protein Tob4, Protein Tob2
计算分子量 344 aa, 37 kDa
观测分子量 43 kDa
GenBank蛋白编号BC038957
基因名称 TOB2
Gene ID (NCBI) 10766
RRIDAB_2877961
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14106
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TOB2 antibody 13607-1-APDownload protocol
IHC protocol for TOB2 antibody 13607-1-APDownload protocol
IF protocol for TOB2 antibody 13607-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Virol

SARS-CoV-2 disrupts proximal elements in the JAK-STAT pathway.

Authors - Da-Yuan Chen
View Article
humanWB

bioRxiv

SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.

Authors - Da-Yuan Chen
View Article