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  • Featured Product
  • KD/KO Validated

TOLLIP Monoclonal antibody, PBS Only

TOLLIP Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B3C4

Cat no : 68170-1-PBS

Print datasheet

Synonyms

Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 1B3C4

验证数据展示

  • WB analysis using 68170-1-Ig (same clone as 68170-1-PBS)

    WB analysis using 68170-1-Ig (same clone as 68170-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68170-1-Ig (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

  • WB analysis using 68170-1-Ig (same clone as 68170-1-PBS)

    WB analysis using 68170-1-Ig (same clone as 68170-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68170-1-Ig (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 68170-1-Ig (same clone as 68170-1-PBS)

    WB analysis of HepG2 using 68170-1-Ig (same clone as 68170-1-PBS)

    WB result of TOLLIP antibody (68170-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOLLIP transfected HepG2 cells. This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 68170-1-Ig (same clone as 68170-1-PBS)

    IHC staining of mouse colon using 68170-1-Ig (same clone as 68170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TOLLIP antibody included
  • IHC staining of mouse colon using 68170-1-Ig (same clone as 68170-1-PBS)

    IHC staining of mouse colon using 68170-1-Ig (same clone as 68170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TOLLIP antibody included
  • IHC staining of rat colon using 68170-1-Ig (same clone as 68170-1-PBS)

    IHC staining of rat colon using 68170-1-Ig (same clone as 68170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TOLLIP antibody included
  • IF Staining of U2OS using 68170-1-Ig (same clone as 68170-1-PBS)

    IF Staining of U2OS using 68170-1-Ig (same clone as 68170-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using TOLLIP antibody (68170-1-Ig, Clone: 1B3C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 68170-1-Ig (same clone as 68170-1-PBS)

    FC experiment of Jurkat using 68170-1-Ig (same clone as 68170-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human TOLLIP (68170-1-Ig, Clone:1B3C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TOLLIP Ready-To-Use IHC Kit. TOLLIP primary antibody included.

产品信息

68170-1-PBS targets TOLLIP in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen TOLLIP fusion protein Ag32731 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name toll interacting protein
Synonyms Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 1B3C4
Calculated Molecular Weight 30 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC012057
Gene Symbol TOLLIP
Gene ID (NCBI) 54472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H0E2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).