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TOLLIP Recombinant antibody

TOLLIP Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242098C6

Cat no : 84711-6-RR

Print datasheet

Synonyms

Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 242098C6

验证数据展示

  • WB analysis using 84711-6-RR

    WB analysis using 84711-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84711-6-RR (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84711-6-RR

    Affinity and Kinetic Characterization of 84711-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84711-6-RR against Human TOLLIP were performed. The affinity constant is 1.32 nM.

经过测试的应用

Positive WB detected inLNCaP cells, HepG2 cells, PC-3 cells, HCT 116 cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy TOLLIP Ready-To-Use IHC Kit. TOLLIP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84711-6-RR targets TOLLIP in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen TOLLIP fusion protein Ag1864 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name toll interacting protein
Synonyms Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 242098C6
Calculated Molecular Weight 30 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC012057
Gene Symbol TOLLIP
Gene ID (NCBI) 54472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9H0E2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).

实验方案

Product Specific Protocols
WB protocol for TOLLIP antibody 84711-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols