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验证数据展示

  • WB analysis using 84711-6-RR

    WB analysis using 84711-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84711-6-RR (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84711-6-RR

    Affinity and Kinetic Characterization of 84711-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84711-6-RR against Human TOLLIP were performed. The affinity constant is 1.32 nM.

TOLLIP Recombinant antibody

TOLLIP Recombinant Antibody for WB, ELISA
Cat No. 84711-6-RR

产品说明书

CloneNo. 242098C6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 242098C6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HepG2 cells, PC-3 cells, HCT 116 cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy TOLLIP Ready-To-Use IHC Kit. TOLLIP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84711-6-RR targets TOLLIP in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 TOLLIP fusion protein Ag1864 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 toll interacting protein
别名 Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 242098C6
计算分子量 30 kDa
观测分子量30 kDa
GenBank蛋白编号BC012057
基因名称 TOLLIP
Gene ID (NCBI) 54472
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H0E2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).

实验方案

Product Specific Protocols
WB protocol for TOLLIP antibody 84711-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols