• Featured Product
  • KD/KO验证

TOMM40 Monoclonal antibody

TOMM40 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66658-1-Ig

产品说明书

CloneNo. 5E2C5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA and More (1)

TOM40, 5E2C5, C19orf1, D19S1177E, p38.5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, human brain tissue, pig brain tissue, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW264.7 cells, K-562 cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66658-1-Ig targets TOMM40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat
免疫原 TOMM40 fusion protein Ag13065 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 translocase of outer mitochondrial membrane 40 homolog (yeast)
别名 TOM40, 5E2C5, C19orf1, D19S1177E, p38.5
计算分子量 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC017224
基因名称 TOMM40
Gene ID (NCBI) 10452
RRIDAB_2882015
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO96008
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TOMM40 antibody 66658-1-IgDownload protocol
IHC protocol for TOMM40 antibody 66658-1-IgDownload protocol
IF protocol for TOMM40 antibody 66658-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Science

Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.

Authors - Qiang Wang
humanWB

Mol Cell

Dynamic Regulation of Mitochondrial Import by the Ubiquitin System.

Authors - Lilian Phu
mouseWB,IF

Cell Death Differ

Tufm lactylation regulates neuronal apoptosis by modulating mitophagy in traumatic brain injury

Authors - Weiji Weng
humanWB

Elife

Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stress

Authors - Jinyang Wang
mouseWB

Sci Signal

A genome-wide screen uncovers multiple roles for mitochondrial nucleoside diphosphate kinase D in inflammasome activation.

Authors - Orna Ernst
ratWB

Apoptosis

Lactate accelerates vascular calcification through NR4A1-regulated mitochondrial fission and BNIP3-related mitophagy.

Authors - Yi Zhu
Loading...