• Featured Product
  • KD/KO Validated

TOMM40 Monoclonal antibody

TOMM40 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (3)

Applications

WB, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

5E2C5

Cat no : 66658-1-Ig

Print datasheet

Synonyms

C19orf1, D19S1177E, p38.5, PER EC1, PEREC1, Protein Haymaker, TOM40, TOMM40



经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, human brain tissue, pig brain tissue, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW264.7 cells, K-562 cells, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHepG2 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66658-1-Ig targets TOMM40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityhuman, mouse, rat
Immunogen TOMM40 fusion protein Ag13065 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name translocase of outer mitochondrial membrane 40 homolog (yeast)
Synonyms C19orf1, D19S1177E, p38.5, PER EC1, PEREC1, Protein Haymaker, TOM40, TOMM40
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC017224
Gene Symbol TOMM40
Gene ID (NCBI) 10452
RRIDAB_2882015
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO96008
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TOMM40 antibody 66658-1-IgDownload protocol
IF protocol for TOMM40 antibody 66658-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Science

Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.

Authors - Qiang Wang
humanWB

Mol Cell

Dynamic Regulation of Mitochondrial Import by the Ubiquitin System.

Authors - Lilian Phu
mouseWB,IF

Cell Death Differ

Tufm lactylation regulates neuronal apoptosis by modulating mitophagy in traumatic brain injury

Authors - Weiji Weng
humanWB

Elife

Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stress

Authors - Jinyang Wang
mouseWB

Sci Signal

A genome-wide screen uncovers multiple roles for mitochondrial nucleoside diphosphate kinase D in inflammasome activation.

Authors - Orna Ernst
ratWB

Apoptosis

Lactate accelerates vascular calcification through NR4A1-regulated mitochondrial fission and BNIP3-related mitophagy.

Authors - Yi Zhu