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TOMM6 Polyclonal antibody

TOMM6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16689-1-AP

Print datasheet

Synonyms

OBTP, TOM6, TOMM6

验证数据展示

  • IHC staining of human breast cancer using 16689-1-AP

    IHC staining of human breast cancer using 16689-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16689-1-AP (TOMM6 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 16689-1-AP

    IHC staining of human breast cancer using 16689-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16689-1-AP (TOMM6 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human skeletal muscle using 16689-1-AP

    WB analysis of human skeletal muscle using 16689-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16689-1-AP (TOMM6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16689-1-AP targets TOMM6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TOMM6 fusion protein Ag10037 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name translocase of outer mitochondrial membrane 6 homolog (yeast)
Synonyms OBTP, TOM6, TOMM6
Calculated Molecular Weight 74 aa, 8 kDa
Observed Molecular Weight 8 kDa
GenBank Accession NumberBC018624
Gene Symbol TOMM6
Gene ID (NCBI) 100188893
RRIDAB_2240919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96B49
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOMM6, also named as OBTP and TOM6, is a part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex).

实验方案

Product Specific Protocols
WB protocol for TOMM6 antibody 16689-1-APDownload protocol
IHC protocol for TOMM6 antibody 16689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols