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  • Featured Product
  • KD/KO Validated

TOP2A Monoclonal antibody, PBS Only

TOP2A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E12B3

Cat no : 66541-1-PBS

Print datasheet

Synonyms

DNA topoisomerase 2 alpha, TOP2, TOP2A, TP2A

验证数据展示

  • WB analysis using 66541-1-Ig (same clone as 66541-1-PBS)

    WB analysis using 66541-1-Ig (same clone as 66541-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66541-1-Ig (TOP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66541-1-Ig (same clone as 66541-1-PBS)

    WB analysis of HepG2 using 66541-1-Ig (same clone as 66541-1-PBS)

    WB result of TOP2A antibody (66541-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A transfected HepG2 cells. This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66541-1-Ig (same clone as 66541-1-PBS)

    IHC staining of human lung cancer using 66541-1-Ig (same clone as 66541-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66541-1-Ig (TOP2A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66541-1-Ig (same clone as 66541-1-PBS)

    IF Staining of HepG2 using 66541-1-Ig (same clone as 66541-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66541-1-Ig (TOP2A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

产品信息

66541-1-PBS targets TOP2A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen TOP2A fusion protein Ag19551 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name topoisomerase (DNA) II alpha 170kDa
Synonyms DNA topoisomerase 2 alpha, TOP2, TOP2A, TP2A
Calculated Molecular Weight 1531 aa, 174 kDa
Observed Molecular Weight 174 kDa
GenBank Accession NumberBC140791
Gene Symbol TOP2A
Gene ID (NCBI) 7153
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11388
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.