验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HL-60 cells |
| Positive IP detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14525-1-AP targets TOP3A in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, xenopus |
| 免疫原 | TOP3A fusion protein Ag6010 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | topoisomerase (DNA) III alpha |
| 别名 | DNA topoisomerase 3 alpha, DNA topoisomerase III alpha, TOP3, TOP3A, topoisomerase (DNA) III alpha |
| 计算分子量 | 1001 aa, 112 kDa |
| 观测分子量 | 100-110 kDa |
| GenBank蛋白编号 | BC051748 |
| 基因名称 | TOP3A |
| Gene ID (NCBI) | 7156 |
| RRID | AB_2205881 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13472 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNA topoisomerase 3-alpha (TOP3A) is an essential component of the RMI complex, a complex involves in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. It releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. This antibody is specific to react with the 110kd human TOP3A.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TOP3A antibody 14525-1-AP | Download protocol |
| IP protocol for TOP3A antibody 14525-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell BLM and BRCA1-BARD1 coordinate complementary mechanisms of joint DNA molecule resolution | ||
Mol Cell TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation. | ||
Mol Cell Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.
| ||
Sci Adv The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication. | ||
Nat Commun The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks. | ||
