验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HEK-293 cells, mouse kidney tissue, mouse liver tissue, rat brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
10296-1-AP targets Torsin A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | Torsin A fusion protein Ag0358 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | torsin family 1, member A (torsin A) |
| 别名 | DQ2, Dystonia 1 protein, DYT1, TOR1A, Torsin 1A, Torsin A, Torsin family 1 member A, TorsinA |
| 计算分子量 | 38 kDa |
| 观测分子量 | 42-50 kDa |
| GenBank蛋白编号 | BC000674 |
| 基因名称 | TOR1A |
| Gene ID (NCBI) | 1861 |
| RRID | AB_2206116 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14656 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TOR1A (torsin A), also known as DYT1, is a member of AAA+ family of ATPases that mediate chaperone and other functions involved in conformational modeling of proteins, protection from stress, and targeting of proteins to cellular organelles. TOR1A is a 37 kDa ATP binding protein highly expressed in hippocampus, spinal cord, kidney and liver. TOR1A primarily localizes to the ER while exposure to oxidative stress causes the redistribution to cell surface protrusions. Mutations in gene encoding TOR1A cause the most severe form of hereditary dystonia, termed early onset, generalized dystonia. Interestingly, Lewy bodies of PD patients were intensely immunoreactive for torsinA.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Torsin A antibody 10296-1-AP | Download protocol |
| IHC protocol for Torsin A antibody 10296-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
