Torsin A Polyclonal antibody
Torsin A Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HEK-293 cells, mouse kidney tissue, mouse liver tissue, rat brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
10296-1-AP targets Torsin A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | Torsin A fusion protein Ag0358 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | torsin family 1, member A (torsin A) |
| Synonyms | DQ2, Dystonia 1 protein, DYT1, TOR1A, Torsin 1A, Torsin A, Torsin family 1 member A, TorsinA |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 42-50 kDa |
| GenBank Accession Number | BC000674 |
| Gene Symbol | TOR1A |
| Gene ID (NCBI) | 1861 |
| RRID | AB_2206116 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14656 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TOR1A (torsin A), also known as DYT1, is a member of AAA+ family of ATPases that mediate chaperone and other functions involved in conformational modeling of proteins, protection from stress, and targeting of proteins to cellular organelles. TOR1A is a 37 kDa ATP binding protein highly expressed in hippocampus, spinal cord, kidney and liver. TOR1A primarily localizes to the ER while exposure to oxidative stress causes the redistribution to cell surface protrusions. Mutations in gene encoding TOR1A cause the most severe form of hereditary dystonia, termed early onset, generalized dystonia. Interestingly, Lewy bodies of PD patients were intensely immunoreactive for torsinA.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Torsin A antibody 10296-1-AP | Download protocol |
| IHC protocol for Torsin A antibody 10296-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neurobiol Dis Postnatal Foxp2 regulates early psychiatric-like phenotypes and associated molecular alterations in the R6/1 transgenic mouse model of Huntington's disease |
