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TOR1AIP1 Polyclonal antibody

TOR1AIP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 21459-1-AP

Print datasheet

Synonyms

LAP1B, LAP1, Torsin-1A-interacting protein 1, TOR1AIP 1, LGMD2Y

验证数据展示

  • WB analysis using 21459-1-AP

    WB analysis using 21459-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21459-1-AP (TOR1AIP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 21459-1-AP

    IF Staining of HeLa using 21459-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOR1AIP1 antibody (21459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, HEK-293T cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21459-1-AP targets TOR1AIP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen TOR1AIP1 fusion protein Ag14823 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name torsin A interacting protein 1
Synonyms LAP1B, LAP1, Torsin-1A-interacting protein 1, TOR1AIP 1, LGMD2Y
Calculated Molecular Weight462aa,52 kDa; 583aa,66 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC023247
Gene Symbol TOR1AIP1
Gene ID (NCBI) 26092
RRIDAB_2878861
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JTV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TOR1AIP1 antibody 21459-1-APDownload protocol
IF protocol for TOR1AIP1 antibody 21459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Elife

CDK1 controls CHMP7-dependent nuclear envelope reformation.

Authors - Alberto T Gatta
View Article
mouse,humanWB,IF,IP

Biochem Biophys Res Commun

Analysis of the GFP-labelled β-dystroglycan interactome in HEK-293 transfected cells reveals novel intracellular networks

Authors - Francesca Sciandra
View Article
humanWB,IF

Nat Cell Biol

LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion

Authors - Yaiza Jung-Garcia
View Article