验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, rat liver tissue, MCF-7 cells, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21162-1-AP targets TOX2 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IF, ChIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | TOX2 fusion protein Ag14772 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | TOX high mobility group box family member 2 |
| Synonyms | GCX-1, GCX1, C20orf100 |
| Calculated Molecular Weight | 488 aa, 52 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC007636 |
| Gene Symbol | TOX2 |
| Gene ID (NCBI) | 84969 |
| RRID | AB_2878821 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96NM4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TOX2 antibody 21162-1-AP | Download protocol |
| IHC protocol for TOX2 antibody 21162-1-AP | Download protocol |
| IP protocol for TOX2 antibody 21162-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Tox2 is required for the maintenance of GC TFH cells and the generation of memory TFH cells.
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Mol Cancer Super-enhancer-driven TOX2 mediates oncogenesis in Natural Killer/T Cell Lymphoma
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