验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, rat liver tissue, MCF-7 cells, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21162-1-AP targets TOX2 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | TOX2 fusion protein Ag14772 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TOX high mobility group box family member 2 |
| 别名 | GCX-1, GCX1, C20orf100 |
| 计算分子量 | 488 aa, 52 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC007636 |
| 基因名称 | TOX2 |
| Gene ID (NCBI) | 84969 |
| RRID | AB_2878821 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96NM4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TOX2 antibody 21162-1-AP | Download protocol |
| IHC protocol for TOX2 antibody 21162-1-AP | Download protocol |
| IP protocol for TOX2 antibody 21162-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Tox2 is required for the maintenance of GC TFH cells and the generation of memory TFH cells.
| ||
Mol Cancer Super-enhancer-driven TOX2 mediates oncogenesis in Natural Killer/T Cell Lymphoma
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