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验证数据展示

  • WB analysis using 30474-1-AP

    WB analysis using 30474-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30474-1-AP (TOX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 30474-1-AP

    IF Staining of A431 using 30474-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TOX2 antibody (30474-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

TOX2 Polyclonal antibody

TOX2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30474-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

GCX-1, GCX1, C20orf100

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30474-1-AP targets TOX2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TOX2 fusion protein Ag31226 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TOX high mobility group box family member 2
别名 GCX-1, GCX1, C20orf100
计算分子量 488 aa, 52 kDa
观测分子量70 kDa
GenBank蛋白编号BC007636
基因名称 TOX2
Gene ID (NCBI) 84969
RRIDAB_3086330
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96NM4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).

实验方案

Product Specific Protocols
WB protocol for TOX2 antibody 30474-1-APDownload protocol
IF protocol for TOX2 antibody 30474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols