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TP53BP1-Specific Recombinant antibody

TP53BP1-Specific Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240638C6

Cat no : 83809-1-RR

Print datasheet

Synonyms

TP53BP1, p53-binding protein 1, p53 binding protein 1, p202, FLJ41424

验证数据展示

  • WB analysis using 83809-1-RR

    WB analysis using 83809-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83809-1-RR (TP53BP1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83809-1-RR

    IF Staining of HeLa using 83809-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TP53BP1-Specific antibody (83809-1-RR, Clone: 240638C6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83809-1-RR

    FC experiment of HeLa using 83809-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TP53BP1-Specific Recombinant antibody (83809-1-RR, Clone:240638C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83809-1-RR targets TP53BP1-Specific in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor protein p53 binding protein 1
Synonyms TP53BP1, p53-binding protein 1, p53 binding protein 1, p202, FLJ41424
Calculated Molecular Weight 214 kDa
Observed Molecular Weight450 kDa
GenBank Accession NumberNM_005657
Gene Symbol TP53BP1
Gene ID (NCBI) 7158
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ12888
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TP53BP1, also named as p53BP1, p202, 53BP1 and FLJ41424, may have a role in checkpoint signaling during mitosis. TP53BP1 enhances TP53-mediated transcriptional activation. TP53BP1 plays a role in the response to DNA damage.

实验方案

Product Specific Protocols
WB protocol for TP53BP1-Specific antibody 83809-1-RRDownload protocol
IF protocol for TP53BP1-Specific antibody 83809-1-RRDownload protocol
FC protocol for TP53BP1-Specific antibody 83809-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols