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TPBG Polyclonal antibody

TPBG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29394-1-AP

Print datasheet

Synonyms

5T4, 5T4 AG, 5T4 oncofetal antigen, M6P1, TPBG, trophoblast glycoprotein

验证数据展示

  • WB analysis using 29394-1-AP

    WB analysis using 29394-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29394-1-AP (TPBG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 29394-1-AP

    IHC staining of mouse eye using 29394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 29394-1-AP (TPBG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

29394-1-AP targets TPBG in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity Human, rat, mouse
Cited Reactivityhuman
Immunogen TPBG fusion protein Ag30019 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name trophoblast glycoprotein
Synonyms 5T4, 5T4 AG, 5T4 oncofetal antigen, M6P1, TPBG, trophoblast glycoprotein
Calculated Molecular Weight 46 kDa
Observed Molecular Weight72-80 kDa
GenBank Accession NumberNM_006670
Gene Symbol TPBG
Gene ID (NCBI) 7162
RRIDAB_2918290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13641
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Trophoblast glycoprotein (TPBG), also termed 5T4, is a 72 kDa transmembrane glycoprotein and an extensively investigated oncofetal antigen, which is limited in normal adult tissues, but highly expressed in various types of cancer in human. TPBG is a novel PKCa-dependent phosphoprotein in retinal rod bipolar cells (RBCs). (PMID: 18097562, PMID: 31255707)

实验方案

Product Specific Protocols
WB protocol for TPBG antibody 29394-1-APDownload protocol
IHC protocol for TPBG antibody 29394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Manag Res

MIR31HG Expression Predicts Poor Prognosis and Promotes Colorectal Cancer Progression.

Authors - Jianlong Wang
View Article