验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, MCF-7 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
29394-1-AP targets TPBG in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | Human, rat, mouse |
| 文献引用反应性 | human |
| 免疫原 | TPBG fusion protein Ag30019 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | trophoblast glycoprotein |
| 别名 | 5T4, 5T4 AG, 5T4 oncofetal antigen, M6P1, TPBG, trophoblast glycoprotein |
| 计算分子量 | 46 kDa |
| 观测分子量 | 72-80 kDa |
| GenBank蛋白编号 | NM_006670 |
| 基因名称 | TPBG |
| Gene ID (NCBI) | 7162 |
| RRID | AB_2918290 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13641 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Trophoblast glycoprotein (TPBG), also termed 5T4, is a 72 kDa transmembrane glycoprotein and an extensively investigated oncofetal antigen, which is limited in normal adult tissues, but highly expressed in various types of cancer in human. TPBG is a novel PKCa-dependent phosphoprotein in retinal rod bipolar cells (RBCs). (PMID: 18097562, PMID: 31255707)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TPBG antibody 29394-1-AP | Download protocol |
| IHC protocol for TPBG antibody 29394-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
