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TPD52 Polyclonal antibody

TPD52 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27648-1-AP

Print datasheet

Synonyms

D52, hD52, N8L, PC 1, PrLZ, Protein N8, TPD52, tumor protein D52

验证数据展示

  • WB analysis using 27648-1-AP

    WB analysis using 27648-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27648-1-AP (TPD52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 27648-1-AP

    IHC staining of human stomach cancer using 27648-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27648-1-AP (TPD52 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPD52 antibody included
  • IHC staining of human stomach cancer using 27648-1-AP

    IHC staining of human stomach cancer using 27648-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27648-1-AP (TPD52 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPD52 antibody included

经过测试的应用

Positive WB detected inL02 cells, HepG2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TPD52 Ready-To-Use IHC Kit. TPD52 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27648-1-AP targets TPD52 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen TPD52 fusion protein Ag26503 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor protein D52
Synonyms D52, hD52, N8L, PC 1, PrLZ, Protein N8, TPD52, tumor protein D52
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 23-27 kDa
GenBank Accession NumberBC018117
Gene Symbol TPD52
Gene ID (NCBI) 7163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55327
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TPD52 antibody 27648-1-APDownload protocol
IHC protocol for TPD52 antibody 27648-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols