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验证数据展示

  • WB analysis using 27648-1-AP

    WB analysis using 27648-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27648-1-AP (TPD52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 27648-1-AP

    IHC staining of human stomach cancer using 27648-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27648-1-AP (TPD52 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPD52 antibody included
  • IHC staining of human stomach cancer using 27648-1-AP

    IHC staining of human stomach cancer using 27648-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27648-1-AP (TPD52 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPD52 antibody included

TPD52 Polyclonal antibody

TPD52 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27648-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

D52, hD52, N8L, PC 1, PrLZ, Protein N8, TPD52, tumor protein D52

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, HepG2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TPD52 Ready-To-Use IHC Kit. TPD52 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27648-1-AP targets TPD52 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 TPD52 fusion protein Ag26503 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor protein D52
别名 D52, hD52, N8L, PC 1, PrLZ, Protein N8, TPD52, tumor protein D52
计算分子量 24 kDa
观测分子量 23-27 kDa
GenBank蛋白编号BC018117
基因名称 TPD52
Gene ID (NCBI) 7163
RRIDAB_3085979
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55327
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TPD52 antibody 27648-1-APDownload protocol
IHC protocol for TPD52 antibody 27648-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols