验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14732-1-AP targets hD53; TPD52L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | hD53; TPD52L1 fusion protein Ag6493 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor protein D52-like 1 |
| 别名 | TPD53, TPD52L1, Tumor protein D52-like 1 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC002375 |
| 基因名称 | TPD52L1 |
| Gene ID (NCBI) | 7164 |
| RRID | AB_2272159 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16890 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tumor protein D52-like 1 (TPD52L1) gene, also known as hD53, encodes a member of the tumor protein D52 (TPD52) family. TPD52-like proteins are coiled-coil motif-bearing proteins first identified through their expression in human breast carcinoma, which have been proposed to represent signaling intermediates and regulators of vesicle trafficking. hD53 may form homo- or heterodimers with other TPD52 family members, and it is reported to be involved in cell proliferation and calcium signaling. Four variants resulted from alternative splicing have been predicated and this antibody is expected to recognize all the variants.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for hD53; TPD52L1 antibody 14732-1-AP | Download protocol |
| IHC protocol for hD53; TPD52L1 antibody 14732-1-AP | Download protocol |
| IF protocol for hD53; TPD52L1 antibody 14732-1-AP | Download protocol |
| IP protocol for hD53; TPD52L1 antibody 14732-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Biosci Tumor protein D52 is upregulated in oral squamous carcinoma cells under hypoxia in a hypoxia-inducible-factor-independent manner and is involved in cell death resistance. | ||
Front Oncol Transcriptomic Analyses of the Adenoma-Carcinoma Sequence Identify Hallmarks Associated With the Onset of Colorectal Cancer. | ||
Int J Oncol Opposite effects of tumor protein D (TPD) 52 and TPD54 on oral squamous cell carcinoma cells. | ||
Biochem J Tumor protein D52 expression is post-transcriptionally regulated by T-cell intercellular antigen (TIA) 1 and TIA-related protein via mRNA stability. | ||
Sci Data The Signaling Pathways Project, an integrated 'omics knowledgebase for mammalian cellular signaling pathways. | ||
Biomedicines Tumor Protein D53 (TPD53): Involvement in Malignant Transformation of Low-Malignant Oral Squamous Cell Carcinoma Cells |